Programme

In a recently completed series of structurefunction studies on human recombinant glycine receptor (GlyR) channels, expressed in HEK293 cells, we have shown that a single, double (SDM) and triple (STM) point mutations in the M2 region of the glycine receptor were able to each switch the GlyR selectivity from being anion to cationselective (Keramidas et al., 2000, 2002; Moorhouse et al., 2002). In order to relate ion selectivity to changes in electrostatic effects in the channel pore and in its minimum pore diameter, we needed both anioncation permeability ratios and minimum pore diameters. The latter were determined by measuring cationcation (or anionanion) permeability ratios for a series of large test cations (anions) for the different cationselective (anionselective) mutant GlyRs. They were determined from bionic potentials, measuring the change in reversal potential, under whole cell patch clamp conditions, when the external solution cation (anion) was substituted by various larger cations (anions) for cation selective (anionselective) mutant GlyRs. It has been shown that for such bionic measurements, the form of the membrane potential equation is essentially independent of the mathematical model underlying it, as discussed in Barry & Gage (1984).
However, concern has been expressed about the validity of using the GoldmanHodgkinKatz (GHK) equation (see Barry & Gage, 1984) for dilution potential measurements, given the inherent assumptions (a constant electrical field in the membrane and independence of ion fluxes) in its derivation. Experimentally, the anioncation permeability ratios (P_{Cl}/P_{Na}) were determined from dilution potentials by measuring the change in reversal potential, when the external solution NaCl concentration was decreased to about 50% and then to about 25% and each shift in reversal potentials plotted against external NaCl activity. Experimentally, it was noted that the data did fit the GHK equation with the predicted straight line and constant permeability ratio. The GHK equation is:
We then fitted the data to the Planck equation, derived by solving the NernstPlanck flux equations, which has virtually opposite underlying assumptions (a nonconstant electrical field and a macroscopic electroneutrality condition) to the GHK ones. The Planck equation is:
Hence, the anioncation permeability ratios determined using the GHK or Planck equations are essentially independent of the limiting underlying assumptions of those equations.
Barry, P.H. & Gage, P.W. (1984) In: Current Topics in Membranes and Transport, 21, ed. Stein, W.E. pp. 151. Orlando: Academic Press.
Keramidas, A., Moorhouse, A.J,. French, C.R., Schofield, P.R. & Barry, P.H. (2000) Biophysical Journal, 78, 247259.
Keramidas, A., Moorhouse, A.J., Pierce, K.D., Schofield, P.R. & Barry P.H. (2002) Journal of General Physiology, 119, 393410.
Moorhouse, A.J., Keramidas, A., Zaykin, A., Schofield, P.R. & Barry P.H. (2002) Journal of General Physiology, 119, 411425.