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Effect of exercise on Ca2+-sensitive protein kinases in human skeletal muscle

A.J. Rose1, B. Michell2, B.E. Kemp2 and M. Hargreaves1, 1Exercise, Muscle & Metabolism Unit, School of Health Sciences, Deakin University, Vic., 3125 and 2St. Vincent's Institute of Medical Research, Fitzroy, Vic., 3065, Australia.

There is evidence in rodents that PKC (Richter et al., 1989; Chen et al., 2002) and CaMKII (Tavi et al., 2003) activities are higher in contracting skeletal muscle, and that these kinases may regulate skeletal muscle function, including metabolism, during exercise. To investigate this in humans, healthy men (n=8, 24 ±5 yr, 23 ±2 kg·m-2, V-dotO2 peak = 51±6 ml·kg-1·min-1) performed cycle ergometer exercise for 40 min at 76±1 % V-dotO2 peak with skeletal muscle samples taken at rest and after 5 and 40 min of exercise. PKC and CaMKII expression and activities were examined by immunoblotting and in vitro kinase assays. There were no differences in maximal (+Ca2+/CaM) CaMKII activity during exercise compared with basal. Autonomous (−Ca2+/CaM) CaMKII activity was 9 ±1% of maximal at rest, unchanged at 5 min, and increased to 17 ±1% (P<0.01) at 40 min. There were no differences in CaMKII expression (P>0.1). There were no changes in cPKC or PKCθ activities (P>0.1), however aPKC activity was ∼70% higher (P<0.05) at 5 and 40 min and total PKC activity was slightly higher at 40 min in an enriched membrane fraction (P<0.05).

The activities of these kinases were also examined in response to maximal aerobic exercise. Healthy men (n=9, 25 ±5 yr, 24 ±2 kg·m-2, 52 ±9 ml·kg-1·min-1) performed cycle ergometer exercise for 10 min at 50 %V-dotO2 peak, after which the workload was increased to elicit 100 %V-dotO2 peak with muscle samples taken at rest and at volitional fatigue. Autonomous CaMKII activity was increased by 74 ±17% (P<0.001) with no change in maximal CaMKII activity. There were no changes in total PKC, PKCδ, PKCθ, or aPKC activities.

These data demonstrate that CaMKII and aPKC are activated in contracting skeletal muscle, and thus may represent key signalling proteins potentially regulating skeletal muscle function and metabolism during exercise in humans.

Chen, H.C., Bandyopadhyay, G., Sajan, M.P., Kanoh, Y., Standaert, M., Farese, R.V. Jr. & Farese, R.V. (2002) Journal of Biological Chemistry, 277, 23554-23562.

Richter, E.A., Cleland, P.J., Rattigan, S., Clark, M.G. (1989) FEBS Letters, 217, 232-236.

Tavi, P., Allen, D.G., Niemela, P., Vuolteenaho, O., Weckstrom, M. & Westerblad, H. (2003) Journal of Physiology, in press.