The precise control of contraction in skeletal muscle critically depends on the rapid and precise delivery of Ca2+ from the specialized internal store, the sarcoplasmic reticulum (SR), which is under voltage control from the tightly apposed tubular (t-) system. Regulation of Ca2+ in the SR and cytoplasm during periods of muscle work is complex and involves influxes of Ca2+ from the t-system. A major problem is that measurements of voltage and store-dependent Ca2+ fluxes from the t-system of skeletal muscle are not possible with standard electrophysiological techniques. Methods to simultaneously image Ca2+ in two subcellular compartments of single muscle fibres using laser scanning confocal microscopy have recently been developed. This has largely involved trapping one Ca2+ sensitive dye in the t-system of a mechanically skinned fibre and introducing a second spectrally separate Ca2+ indicator to the cytoplasm. By stimulating release of Ca2+ directly from SR or via t-system depolarization, voltage dependent and independent fluxes across the t-system can be spatiotemporally resolved against the release flux of Ca2+ from SR. These measurements have identified an action potential-induced Ca2+ flux across the t-system and an ultra-rapid store-operated Ca2+ entry (SOCE) mechanism in skeletal muscle. This has led to the development of a working model for SOCE that is relevant within the large Ca2+ release fluxes initiated by excitation-contraction coupling. Potential roles of the action potential-induced Ca2+ flux in skeletal muscle remain speculative.