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Increased plasma insulin levels during a euglycaemic hyperinsulinaemic clamp (Clerk et al., 2006) or with a mixed meal (carbohydrate, protein and fat) (Vincent et al., 2006) stimulates an increase in microvascular perfusion in skeletal muscle. This microvascular insulin action augments skeletal muscle glucose disposal by increasing the delivery of insulin and glucose to the myocyte. This action is markedly impaired in insulin resistance (Clerk et al., 2006; Keske et al., 2009). The aim of the current study is to determine whether the clinically used oral glucose challenge (OGC) is able to elicit equivalent increases in microvascular perfusion in skeletal muscle as we have previously shown using mixed meal or insulin clamp. Overnight fasted healthy volunteers (49 ± 5 yr, 1F, 7M; mean ± SEM) without a first or second degree relative with type 2 diabetes, underwent an OGC (50g glucose x 1h). Participants had normal: BMI (21.37 ± 2.6), blood glucose (4.7 ± 0.1 mM), plasma insulin (3.6 ± 0.7 mU/L), HbA1c (5.4 ± 0.1 %), total cholesterol (4.8 ± 0.3 mM), LDL (3.1 ± 0.3 mM, calculated using the Friedewald Formula), HDL (1.4 ± 0.1 mM) and triglyceride (0.8 ± 0.2 mM) levels. Following the OGC, circulating glucose increased 1.7-fold
Baseline | 1h OGC | |
Blood glucose (mM) | 4.7 ± 0.1 | 8.1 ± 0.4† |
Brachial artery diameter (mm) | 4.05 ± 0.15 | 4.17 ± 0.18 |
Brachial artery blood flow (ml/min) | 57.3 ± 5.1 | 115.0 ± 26.0* |
Microvascular blood volume (AI) | 1.19 ± 0.26 | 0.94 ± 0.10 |
Microvascular blood flow (AI/s) | 0.14 ± 0.03 | 0.10 ± 0.02* |
Large artery stiffness (cm/s) | 7.18 ± 0.48 | 7.14 ± 0.46 |
Central systolic blood pressure (mmHg) | 116.2 ± 4.8 | 113.1 ± 4.1# |
Central diastolic blood pressure (mmHg) | 79.8 ± 2.9 | 77.3 ± 2.6* |
Clerk LH, Vincent MA, Jahn LA, Liu Z, Lindner JR, Barrett EJ. (2006). Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle. Diabetes 55, 1436-42.
Vincent MA, Clerk LH, Lindner JR, Price WJ, Jahn LA, Leong-Poi H, Barrett EJ. Vincent et al. (2006). Mixed meal and light exercise each recruit muscle capillaries in healthy humans. Am J Physiol Endocrinol Metab 290, E1191-7.
Keske MA, Clerk LH, Price WJ, Jahn LA, Barrett EJ. (2009). Obesity blunts microvascular recruitment in human forearm muscle after a mixed meal. Diabetes Care 32, 1672-7.