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Effect of curcumin exposure on skeletal muscle contractile function

J. Lam, G.J. Pinniger and A.J. Bakker, School of Anatomy, Physiology and Human Biology, University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.

Curcumin, a component of the spice turmeric (Curcuma longa), reportedly alleviates the symptoms of muscular dystrophy in mdx mice (Pan et al., 2008) and decreases the expression of inflammatory mediators involved in muscle injury (Epstein et al., 2012). Based on these observations, curcumin may provide a new avenue for treatment of skeletal muscle damage and wasting. However, curcumin has also been reported to impair Ca2+ handling in sarcoplasmic reticulum (SR) vesicles (Bilmen et al., 2001; Logan-Smith et al., 2001) which could result in curcumin-induced muscle weakness. The aim of this study was to investigate the effects of curcumin exposure on skeletal muscle contractile function using skinned skeletal muscle preparations. Muscle fibres were isolated from the extensor digitorum longus (EDL) muscles of anaethetised ARC mice and Wistar rats (Rattus norvegicus). Mechanically skinned fibres were used to examine the effect of curcumin on excitation-contraction (E-C) coupling and SR function, based on Ca2+ loading and release characteristics. Curcumin (15 μM) reduced SR Ca2+ loading to 75.19 ± 5.29% (SEM) of control levels (P<0.001, n=9) but had no effect on the Ca2+ sensitivity of the contractile apparatus. Curcumin also decreased the peak of depolarisation-induced force (DIF) responses to 52.10 ± 7.97% of controls (P<0.0001, n=13). After curcumin washout, 7 fibres failed to respond to depolarisation with a force response, and in the remaining fibres, DIF responses were reduced to 14.94 ± 6.20% of controls (P<0.0001, n=6) Exposure to a low Mg2+/caffeine Ca2+-release solution shortly after resulted in large force responses similar in peak to control DIF responses (98.75 ± 4.20% of initial control measurements, P=0.78, n=6), indicating that the effects of curcumin on DIF were not due to SR Ca2+ depletion, or inhibition of SR Ca2+ release. The results of this study show that high doses of curcumin inhibit skeletal muscle force production, most likely through inhibitory effects on aspects of E-C coupling upstream from the SR.

Bilmen JG, Khan SZ, Javed M-u-H, Michelangeli F. (2001) Inhibition of the serca Ca2+ pumps by curcumin. Eur J Biochem 268, 6318-6327.

Epstein J, Sanderson IR, MacDonald TT. (2012) Curcumin as a therapeutic agent: The evidence from in vitro, animal and human studies. Br J Nutr 103, 1545-1557.

Logan-Smith MJ, Lockyer PJ, East JM, Lee AG. (2001) Curcumin, a molecule that inhibits the Ca2+-atpase of sarcoplasmic reticulum but increases the rate of accumulation of Ca2+. J Biol Chem 276, 46905-46911.

Pan Y, Chen C, Shen Y, Zhu C-H, Wang G, Wang X-C, Chen H-Q, Zhu M-S. (2008) Curcumin alleviates dystrophic muscle pathology in mdx mice. Mol Cells 25, 531-537.