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PROCEEDINGS OF THE
AUSTRALIAN PHYSIOLOGICAL SOCIETY

Volume 37

2006

Professor Peter Gage
Dedicated to the Memory of
Peter William Gage FAA, DSc, PhD, MB ChB (1937-2005)
Obituary pp 29-30

CONTENTS

AuPS/ASB Canberra 2005 Meeting

AuPS Invited Lecture
Excitation-contraction coupling from the 1950s into the new millennium
A.F. Dulhunty 1
 
Plenary Lecture
Computer simulations of transport through membranes: passive diffusion, pores, channels and transporters
D.P. Tieleman 15
 
Obituary
Professor Peter William Gage FAA, DSc, PhD, MB ChB (1937-2005)
D.J. Adams and P.H. Barry 29
 
Symposium: Membrane Associated Proteins that Regulate Muscle Contraction
Regulation of ryanodine receptors from skeletal and cardiac muscle by components of the cytoplasm and SR lumen during rest and excitation
D.R. Laver 31
Structural and functional characterisation of the interaction of the dihydropyridine receptor II-III loop with the ryanodine receptor
M.G. Casarotto, Y. Cui, Y. Karunasekara, P.J. Harvey, N. Norris, P.G. Board and A.F. Dulhunty 39
ClC-1 chloride channel - matching its properties to a role in skeletal muscle
E.C. Aromataris and G.Y. Rychkov 43
 
Book Rewiew
Review: Case-based Medical Physiology by Christopher Bell, Cecil Kidd & Trefor Morgan
A.E. Sefton 51

AuPS/Combio Brisbane 2006 Abstracts

AuPS Invited Lecture
The developmental origins of adult health and disease 1P
I.C. McMillen, Early Origins of Adult Health Research Group, Sansom Research Institute, University of South Australia, Adelaide, SA 5000, Australia.
Plenary Lectures
Mechanisms of peripheral pain sensitization 2P
G.S. Oxford, Stark Neuroscience Research Institute, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
New insights in age-related impairments in muscle function 3P
J.A. Faulkner, University of Michigan, Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200.
The transient receptor potential (TRP) ion channels: recent progress 4P
D.E. Clapham, Howard Hughes Medical Institute, Department of Cardiology, Children's Hospital Boston, and Department of Neurobiology, Harvard Medical School, Boston, USA.
NHE3 and its signaling complexes: role in acute regulation, cytoskeletal association and protein-protein interactions 5P
M. Donowitz, X. Li, M. Tse and B.Y. Cha, Departments of Physiology and Medicine, Gastroenterology Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Kiss and tell in the brain – kisspeptins and the neural control of fertility 6P-8P
A.E. Herbison, J. Clarkson and R. Campbell, Centre for Neuroendocrinology and Department of Physiology, University of Otago, Dunedin, New Zealand.
Symposium: Myopathies and muscle regeneration
Functional impairments in dystrophic muscles: lateral transmission of force and sarcomere dynamics 9P
J.A. Faulkner, T.Y. Kostrominova and M.L. Palmer, University of Michigan, Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI, USA 48109-2200.
Anti-inflammatory drugs, or increased IGF-1 expression, reduce necrosis of dystrophic muscle 10P
M.D. Grounds,1 H. Radley,1 T. Shavlakadze,1 B. Gebski,1,2 J. Torrisi,1 M. Davies1 and M. Bogoyevitch,2 1School of Anatomy & Human Biology, The University of Western Australia, WA, Australia and 2School of Biomedical & Chemical Sciences, The University of Western Australia, WA, Australia. (Introduced by Gordon Lynch)
Novel filament systems in normal, regenerating and diseased muscle 11P
E.C. Hardeman,1 N. Vlahovich,1 G. Schevzov,2 V. Nair-Shaliker,1 B. Ilkovski,3 D. Hernandaz-Deviez,4 R.G. Parton4 and K.N. North,3,5 1Muscle Development Unit, Children's Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia, 2Oncology Research Unit, The Children's Hospital at Westmead, PO Box 3515, Parramatta, NSW 2145, Australia, 3The Institute for Neuromuscular Research, The Children's Hospital at Westmead, PO Box 3515, Parramatta, NSW 2145, Australia, 4Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, QLD 4072, Australia and 5Discipline of Paediatrics and Child Health, Faculty of Medicine, University of Sydney, Sydney, NSW 4000, Australia. (Introduced by Gordon Lynch.)
Intramuscular injection of the β-agonist formoterol enhances muscle regeneration in rats after myotoxic injury 12P
G.S. Lynch, J.D. Schertzer, T.M. Alabakis, D.R. Plant and J.G. Ryall, Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, Victoria 3010, Australia.
Symposium: The environment and genome in development: signalling from the beginning
Mechanisms of egg activation and how calcium signalling affects embryonic development 13P
K.T. Jones, Institute for Cell and Molecular Biosciences, University of Newcastle-upon-Tyne, NE2 4HH, UK. (Introduced by Jon Curlewis)
Nutrient sensing by the early mouse embryo 14P
M. Pantaleon, School of Biomedical Sciences, The University of Queensland, Brisbane, Qld 4072, Australia. (Introduced by David Adams)
Interactions between fetal programming and postnatal diet: implications for development of the metabolic syndrome 15P
B.J. Waddell, C.S. Wyrwoll, D.P. Hewitt and P.J. Mark, School of Anatomy & Human Biology, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia. (Introduced by Jon Curlewis)
Epigenetic consequences to offspring of nutritional deficiency in pregnancy 16P
M. Dziadek,1,2 G. Konycheva,1 S. Ravelich,1 G. Smith,1 S. Patel1 and M. Ehrich,3 1School of Biological Sciences, University of Auckland, New Zealand, 2Garvan Institute of Medical Research, Darlinghurst, NSW, Australia and 3Sequenom Inc., San Diego, USA. (Introduced by Denis Loiselle)
Symposium: Autonomic Motor System/Smooth Muscle
Control of gut motility 17P
M. Costa, Department of Physiology and Centre of Neuroscience, School of Medicine,
Ion channel dysfunction in endothelium dependent regulation of vascular smooth muscle 18P
H.C. Parkington, A.J. Edgley, R.G. Evans, H.A. Coleman, K.J. Bubb and M. Tare, Department of Physiology, Monash University, Melbourne, Victoria 3800, Australia.
Plasticity of neurovascular transmission following spinal cord injury 19P
J.A. Brock, Prince of Wales Medical Research Institute, Barker St, Randwick, NSW 2031, Australia.
Extrinsic sensory innervation of the gut 20P
S.J.H. Brookes, X.Y. Song, B.N. Chen and M. Costa, Department of Human Physiology, Flinders University, Bedford Park, SA 5042, Australia.
Symposium: Endocrinology
Understanding estrogen feedback actions on the brain using transgenic mouse models 21P
A.E. Herbison, R. Porteous, J. Clarkson and R.E. Campbell, Centre for Neuroendocrinology and Department of Physiology, University of Otago, Dunedin, New Zealand. (Introduced by Jon Curlewis)
Intracellular signals employed by GHS-receptor in the regulation of pituitary growth hormone (GH) secretion 22P
C. Chen, Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Vic 3168, Australia.
Intracellular mechanisms that modulate sensitivity to hormone negative feedback: the regulation of prolactin during lactation 23P
S.T. Anderson,1 J.L. Barclay,1 M.J. Waters1,2 and J.D. Curlewis,1 1School of Biomedical Sciences, and 2Institute of Molecular Bioscience, The University of Queensland, St Lucia, Qld, Australia.
Symposium: Molecular determinants of calcium signalling
Ca2+ signalling and early embryonic patterning during zebrafish development 24P
Sarah E. Webb and Andrew L. Miller, Department of Biology, HKUST, Clear Water Bay, Hong Kong.
Confocal imaging of lumenal and cytosolic [Ca2+] during Ca2+ release in skeletal muscle 25P
B.S. Launikonis1,2 and E. Rios,1 1Rush University, Chicago, IL, USA and 2La Trobe University, Melbourne, Vic., Australia.
The muscle model for intracellular calcium signalling: cross-talk between the calcium release channel and calcium buffer in the intracellular calcium store 26P
A.F. Dulhunty,1 M. Varsanyi,2 L. Wei1 and N.A. Beard,4 1JCSMR, PO Box 334, Canberra City, ACT 2601, Australia and 2Institut für Physiologische Chemie, Ruhr Universität, Bochum, Germany.
Symposium: Development and Physiological Adaptions of Neuronal Circuits and Synapses I
A novel trafficking pathway for NMDA receptors to synapses 27P
J.M. Montgomery1, O. Jeyfrous2,3, T. Desilva1, M. Schubert1, C.G. Garner2 and W.N Green,3 1University of Auckland, New Zealand, 2Stanford University, CA, USA, and 3University of Chicago, IL, USA. (Introduced by John Bekkers)
Neural coding in the primary olfactory cortex 28P
J.M. Bekkers and N. Suzuki, Division of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, ACT 0200, Australia.
Learning rules for spike timing-dependent plasticity depend on dendritic synapse location 29P
J.J. Letzkus,1 B.M. Kampa2 and G.J. Stuart,1 1John Curtin School of Medical Research, Australian National University, ACT 0200, Australia and 2Brain Research Institute, University of Zürich, Switzerland.
SK channels modulate NMDA receptor-mediated synaptic transmission in the lateral amygdala 30P
E.S.L Faber, A.J. Delaney and P. Sah, Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia.
Symposium: Homeostatic control of calcium in the healthy and diseased heart
α1-Adrenergic signaling mediates cardiac adaptation to development and stress 31P
P.C. Simpson, Veteran's Affairs Medical Center, San Francisco, CA, USA and Cardiovascular Research Institute and Department of Medicine, University of California, San Francisco, CA, USA. (Introduced by Livia Hool)
Normal and abnormal functions of stretch-activated channels in the heart 32P
D.G. Allen,1 M.L. Ward2 and I.A. Williams,1 1School of Medical Sciences, University of Sydney, NSW 2006, Australia and 2University of Auckland, Private Bag 92019, Auckland, NZ.
α1A-adrenergic receptors activate phospholipase C, but suppress Ins(1,4,5)P3 generation during ischemia-reperfusion in mouse heart 33P
F. Amirahmadi,1 R.M. Graham2 and E.A. Woodcock,1 1Baker Heart Research Institute, Melbourne, Vic, Australia and 2Victor Chang Cardiac Research Institute, Sydney, NSW, Australia.
Store activation mechanism for cardiac ryanodine receptors 34P
D.R. Laver, School of Biomedical Sciences, University of Newcastle, Callaghan, NSW 2308, Australia, and Hunter Medical Research Institute, Newcastle, NSW, Australia.
Symposium: Development and Physiological Adaptions of Neuronal Circuits and Synapses II
Tissue organization along the neuromuscular axis: laminins match Schwann cell to axon to muscle 35P
B.L. Patton, Oregon Health & Science University, Portland, OR 97239, USA.
MIS and its role in neuronal development 36P
P.Y. Wang, K. Koishi and I.S. McLennan, Neuromuscular Research Group, University of Otago, PO Box 913, Dunedin, New Zealand.
Developmental regulation of the neuromotor circuit: peripheral and central synaptic influences in motoneuron numbers and innervation of muscle 37P
P.G. Noakes,1 K.L. Smallcombe,1 R. Kanjhan,1 A. Arata,2 G. Banks,1 K. Obata,3 H. Betz4 and M.C. Bellingham,1 1Synaptic Biology Group, School of Biomedical Sciences, University of Queensland, St Lucia, Qld, Australia, 2Laboratory for Memory and Learning, Riken Brain Science Institute, Wako, Japan, 3Obata Research Unit, Riken Brain Science Institute, Wako, Japan and 4Department of Neurochemistry, Max Planck Institute for Brain Research, Frankfurt/Main, Germany.
Symposium: Regulation of membrane transport
Role of proteases in the activation of epithelial Na+ channels 38P
T.R. Kleyman and R.P. Hughey, Department of Medicine, University of Pittsburgh, Pittsburg, PA, USA. (Introduced by David Cook)
Evidence for the regulation of ion channels in the heart by reactive oxygen species – mechanism for mediating pathology 39P
L.C. Hool, Cardiovascular Electrophysiology Laboratory, School of Biomedical Biomolecular and Chemical Sciences, The University of Western Australia, WA, Australia.
Regulation of epithelial sodium channel by Akt and Sgk 40P
I.H. Lee, A. Dinudom and D.I. Cook, Department of Physiology, University of Sydney, NSW, Australia.
Serum- and glucocorticoid-inducible kinase (SGK) interacts with the chloride channel ClC-5 to regulate renal albumin uptake 41P
D.H. Hryciw,1 J. Ekberg,1 A. Lee,1 N. Schimpf,1 S.-J. Conroy,1 C.C. Yun2 and P. Poronnik,1 1School of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, Australia and 2Department of Medicine, Division of Digestive Diseases, Emory University, Atlanta, USA.
Symposium: Novel methodologies for teaching and learning
Ask the audience: using electronic response systems for instant feedback in the classroom 42P
D.U. Silverthorn, University of Texas at Austin, Austin, Texas, USA. (Introduced by ???)
Electronic voting system improves student experience in lectures 43P
K. Farrand, Physiology, School of Molecular & Biomedical Science, University of Adelaide, Adelaide, SA, Australia.
Effective whole class discussions 44P
H. Ernst and K. Colthorpe, School of Biomedical Sciences, University of Queesnland, St Lucia, Qld, Australia.
Using explicit teaching of the opinion editorial to improve communication skills of science students through writing 45P
D.H. Hryciw, P. Poronnik and R.W. Moni, School of Biomedical Sciences, The University of Queensland, St Lucia, QLD 4072, Australia.
Free communications: Regulation of membrane transport – I
The ubiquitin ligase Nedd4-2: a novel regulator of the heteromeric KCNQ2/3 potassium channel 46P
J.A.K. Ekberg,1 S. Conroy,1 F.S. Schuetz,1 N. Boase,2 S. Kumar,2 D.J. Adams1 and P. Poronnik, 1School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia and 2Hanson Institute, IMVS, Frome Road, Adelaide, SA 5000, Australia.
Purinergic regulation of epithelial sodium channels (ENaC) 47P
L.M. O'Mullane and D.I. Cook, Department of Physiology (K25), University of Sydney, NSW 2006, Australia.
PIP2 mediates sodium feedback inhibition in the epithelial sodium channel 48P
C.R. Campbell, A. Dinudom and D.I. Cook, Discipline of Physiology, University of Sydney, NSW 2006, Australia.
Pharmacological function of recombinant human skeletal muscle sodium channels are preserved following ion metal affinity chromatography purification and reconstitution into bilayer lipid membranes 49P
Y.L. Zhang, J.E. Dalziel and J. Dunlop, AgResearch Limited, Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North, New Zealand.
Analysis of function in the carboxyl-terminal domain of the skeletal muscle chloride channel, ClC-1 50P
L.L. Ma,1 G. Rychkov,2 B. Hughes,1 B.J. Roberts,1 S.M. Paltoglou,1 P. Bartley,1 A. Beck1 and A. Bretag,1 1Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia and 2Department of Physiology, University of Adelaide, Adelaide, SA 5000, Australia.
The role of the counter ion in determining the anion-cation permeability ratio in glycine receptor channels of different size 51P
S. Sugiharto,1 A.J. Moorhouse,1 T.M. Lewis,1 P.R. Schofield2 and P.H. Barry,1 1School of Medical Sciences, University of NSW, UNSW Sydney, NSW 2052, Australia and 2Prince of Wales Medical Research Institute, Barker St, Randwick, NSW 2031, Australia.
Free communications: Regulation of membrane transport – II
CLCN7 promoter regulation and disruption of ClC-7 function: potential for osteoporosis therapy 52P
A.H. Bretag,1 P.A. Bartley,1 B. Neumann1 and G.Y. Rychkov,1,2 1Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia and 2Physiology Discipline, University of Adelaide, SA 5005, Australia.
Hyposulfataemia and gastrointestinal physiology 53P
P.A. Dawson,1 N. Strachan,1 S. Huxley,1 M.A. McGuckin2 and D. Markovich,1 1School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia and 2Mater Medical Research Institute, Mater Hospital, South Brisbane, QLD 4101, Australia.
Relationship between gastrin and iron in mice with haemolytic anaemia 54P
S. Kovac, A. Shulkes and G.S. Baldwin, University of Melbourne Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia.
Aspartyl-aminopeptidase regulates albumin endocytosis by interaction with ClC-5 55P
A. Lee,1 D. Hryciw,1 S. Wilk,2 E. Wilk,2 V. Valova,3 P. Robinson3 and P. Poronnik,1 1School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia, 2Department of Pharmacology, Mount Sinai School of Medicine, New York 10029, USA and 3Cell Signalling Unit, Children's Medical Research Institute, Wentworthville, NSW 2145, Australia.
Fungal toxins as pharmacological tools to investigate BK ion channel function 56P
J.E. Dalziel,1 S.F. Finch,2 W.L. Imlach1,3 and J. Dunlop,1 1AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand, 2Ruakura Research Centre, Hamilton, New Zealand and 3University of Otago, Dunedin, New Zealand.
Free communications: Myopathies and muscle regeneration
Effect of L-arginine on cardiac function and fibrosis in mdx mice 57P
A.J. Hoey and C. Van Erp, Department of Biological and Physical Sciences, Centre for Systems Biology, University of Southern Queensland, Toowoomba, QLD 4350, Australia.
Inhibition of β-adrenergic signalling impairs functional repair of rat skeletal muscle after injury 58P
F. Beitzel,1 M.N. Sillence2 and G.S. Lynch,1 1Basic and Clinical Myology Laboratory, Department of Physiology, University of Melbourne, Victoria 3010, Australia and 2School of Agriculture, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia.
Effect of protease-activated receptor stimulation on electrically evoked calcium transients elicited in cultured skeletal C2C12 myotubes 59P
A.J. Bakker, G. Ravenscroft and L. Van Kann, Physiology, School of Biomedical, Biomolecular & Chemical Sciences, University of Western Australia, Perth, WA 6009, Australia.
Calcineurin activation can enhance the structure and function of regenerating muscles following myotoxic injury 60P
N. Stupka,1 J.D. Schertzer,1 R. Basel-Duby,2 E.N. Olson2 and G.S. Lynch,1 1Department of Physiology, The University of Melbourne, Melbourne, Vic, Australia, and 2University of Texas Southwestern Medical Center, Dallas, TX, USA.
Formoterol-induced hypertrophy of slow-twitch skeletal and cardiac muscle is reversible after withdrawal of treatment 61P
J.G. Ryall, J.D. Schertzer and G.S. Lynch, Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, Victoria 3010, Australia.
Protein synthesis measurements in mechanically skinned skeletal muscle fibres of the rat 62P
D.W. Jame, M. Jois and D.G. Stephenson, La Trobe University, Victoria 3086, Australia.
Free communications: Calcium Signalling
Calcium signalling in glial cells: a potential role for TRP channels 63P
K.L. Richardson,1 C.A. Reid,2 K. Powell,3 M. Monif1 and D.A. Williams,1 1Department of Physiology, The University of Melbourne, Vic, Australia, 2Howard Florey Institute, Melbourne, Vic, Australia and 3Department of Medicine, The University of Melbourne, Vic, Australia.
The role of different isoforms of IP3 receptor in activation of store-operated Ca2+ channels in liver cells 64P
L. Jones,1 T. Litjens,1 M. Roberts,1 G. Barritt2 and G. Rychkov,1 1School of Molecular and Biomedical Science, University of Adelaide, SA, Australia and 2School of Medicine, Flinders University of South Australia, SA, Australia.
A role for secretory pathway calcium ATPase 2 (SPCA2) in calcium transport, breast cancer and mammary gland development 65P
H. Faddy,1 C. Smart,2 M.A. Brown,2 S. Roberts-Thomson1 and G. Monteith,1 1School of Pharmacy, The University of Queensland, Brisbane, QLD, 4072, Australia and 2School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
Muscarinic agonist-induced recruitment of plasma membrane Ca2+ ATPase (PMCA) to the membrane involves the PSD95/Dlg/ZO-1 (PDZ) scaffold Na+ H+ exchanger regulatory factor 2 (NHERF-2) 66P
W.A. Kruger,1 G.R. Monteith2 and P. Poronnik,1 1School of Biomedical Science and 2School of Pharmacy, University of Queensland, St Lucia, Qld 4072, Australia.
Serine proteases as initiators of intracellualr signalling 67P
J.D. Hooper, A.J. Ramsay, J.P. Quigley, J.C. Reid, and M.L. Hunt, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4001, Australia.
Altered contractile, electrophysiological and Ca2+ release from left atria and isolated ventricular myocytes from mdx mice 68P
G. Marrett, N. Laws, M. Watson and A. Hoey, University of Southern Queensland, Biological & Physical Sciences, Centre for Systems Biology, Toowoomba, QLD 4350, Australia.
Free communications: ANS/Smooth Muscle
Increased levels of TRPC1 and caveolin-3 in mdx mouse 69P
O.L. Gevasio, N.P. Whitehead and D.G. Allen, School of Medical Sciences (F13), Bosch Institute, The University of Sydney, NSW 2006, Australia.
Pyeloureteric peristalsis: role of interstitial cells of Cajal (ICC)-like cells as secondary pacemakers 70P
R.J. Lang, M.A. Tonta, B. Zoltkowski, I. Wendt and H.C. Parkington, Depatment of Physiology, Monash University, Clayton, Vic 3800, Australia.
Adaptations following partial denervation of the rat tail artery maintain normal neurovascular control 71P
D. Tripovic, S. Pianova, E.M. McLachlan and J.A. Brock, Prince of Wales Medical Research Institute, Barker St, Randwick, NSW 2031, Australia.
Autonomic control of airways vascular and wall smooth muscle during exercise 72P
S.W. White,1,2 D. McLeod,1,2 G. Parsons,3 R. Gunther,4 S.A. McIlveen,1,2 R. Bishop,1,2 A.W. Quail1,2 and D.B. Cottee,1,2 1School of Biomedical Sciences, University of Newcastle, NSW 2308, Australia, 2Hunter Medical Research Institute, John Hunter Hospital, New Lambton, NSW 2305, Australia, 3Division of Pulmonary and Critical Care Medicine, University of California Davis Medical Center, Stockton Blvd. Sacramento, CA 95817, USA and 4Division of Surgery, University of California Davis Medical Center, Stockton Blvd, Sacramento, CA 95817, USA.
Graded exercise evokes bronchovascular and lower airway smooth muscle constriction in sheep 73P
D. McLeod,1,2 F. Bastian,1,2 G. Parsons,3 R. Gunther,4 A. Quail,1,2 D. Cottee1,2 and S. White,1,2 1Discipline of Human Physiology, Faculty of Health, University of Newcastle, NSW 2308, Australia, 2Hunter Heart-Lung Research Guild within the Hunter Medical Research Institute, John Hunter Hospital, New Lambton, NSW 2305, Australia, 3Division of Pulmonary and Critical Care Medicine, University of California, Davis, California, 95616, USA and 4Division of Surgery, University of California, Davis, California, 95616, USA.
Free communications: Endocrinology
Linoleic acid induces an increase in intracellular calcium concentration and a membrane hyperpolarization of primary cultured rat pancreatic β-cells 74P
Y.F. Zhao and C. Chen, Endocrine Cell Biology, Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
Dynamics of the fusion pore during exocytosis in mouse pancreatic acinar cells 75P
O. Larina1 and P. Thorn2, 1Department of Physiology, Development & Neuroscience, University of Cambridge, UK and 2School of Biomedical Sciences, University of Queensland, Australia.
Identification of the key binding residues for insulin-like peptide 3 (INSL3) in its receptor, LGR8 76P
D.S. Scott, T. Wilkinson, G.W. Tregear and R.A.D. Bathgate, Howard Florey Institute, University of Melbourne, Vic 3010, Australia.
Retinoid signaling determines germ cell fate in mice 77P
J. Bowles,1 D. Knight,1 C. Smith,1 D. Wilhelm,1 H. Hamada,2 J. Rossant3 and P. Koopman,1 1Institute for Molecular Bioscience, University of Queensland, St Lucia, Qld, Australia, 2Institute for Molecular and Cellular Biology, Osaka University, Osaka, Japan and 3The Hospital for Sick Children, Toronto, Ontario, Canada. (Introduced by Richard Lewis)
Free communications: Development and physiological adaptations of neuronal circuits and synapses
Trafficking by GABARAP produces “super” GABAA channels 78P
M.L. Tierney, P.W. Gage and T. Luu, The John Curtin School of Medical Research, The Australian National University, ACT 0200, Australia.
Postsynaptic packing of acetylcholine receptors at the neuromuscular synapse and its regulation: a FRET study 79P
O.L. Gervasio, P.F. Armson and W.D. Phillips, School of Medical Sciences (Physiology), Bosch Institute, University of Sydney, NSW 2006, Australia.
The role of BK channels in ataxia and tremor produced by fungal toxins 80P
W.L. Imlach,1,3 J.E. Dalziel,1 S.C. Finch2 and J. Dunlop,1 1AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand, 2AgResearch Ltd, Ruakura Research Centre, Hamilton, New Zealand and 3Department of Pharmacology and Toxicology, School of Medical Sciences, University of Otago, Dunedin, New Zealand.
Change in the sensitivity of transmitter release to calcium at β2-laminin deficient nerve terminals 81P
M.P. Schenning, P.G. Noakes and N.A. Lavidis, Synaptic Biology Group, School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia.
Free communications: Posters
Plasma membrane calcium pump (PMCA) expression in a colon cancer cell line during differentiation 82P
C.S. Aung, G.R. Monteith and S.J. Roberts-Thomson, The School of Pharmacy, University of Queensland, Brisbane, QLD 4072, Australia.
P13 protein C-terminal peptide and N-terminal peptide of GBV-B forms ion channels in artificial lipid bilayers 83P
A. Premkumar, B. Wei and P.W. Gage, The John Curtin School of Medical Research, The Australian National University, ACT 0200, Australia.
Transient exposure to extracellular hydrogen peroxide is associated with a persistent increase in intracellular calcium and superoxide from the mitochondria in ventricular myocytes without apoptosis 84P
H.M. Viola, P.G. Arthur and L.C. Hool, School of Biomedical Biomolecular and Chemical Sciences, The University of Western Australia, WA, Australia.
Does the organisation of native GABAA receptors affect their ion channel properties? 85P
V.A.L. Seymour, T. Luu, P.W. Gage and M.L. Tierney, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 0200, Australia.
Dihydropyridines as subunit-specific pharmacological probes of recombinantly expressed glycine receptors 86P
X. Chen and J.W. Lynch, School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia.
Increased acetaminophen hepatotoxicity in the Nas1 and Sat1 sulfate transporter null mice 87P
S. Lee,1 P.A. Dawson,1 A.K. Hewavitharana,2 P.N. Shaw2 and D. Markovich,1 1School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia and 2School of Pharmacy, University of Queensland, St Lucia, QLD 4072, Australia.
Getting active with active learning: an interactive demonstration of neural concepts using the ‘Mexican wave’ 88P
A.L. Parkinson, L.R. Brooker and J. Nayler, Faculty of Science, Health & Education, University of the Sunshine Coast, Queensland 4558, Australia.
IGF-I overexpression in muscles of mdx mice improves excitation-contraction coupling in isolated mechanically skinned muscle fibres 89P
C. Van Der Poel,1 J.D. Schertzer,1 T. Shavlakadze,2 M.D. Grounds2 and G.S. Lynch,1 1Basic and Clinical Myology Laboratory, Department of Physiology, University of Melbourne, Victoria 3010, Australia and 2School of Anatomy and Human Biology, The University of Western Australia, Crawley, Perth, Western Australia 6009, Australia.
Effects of IGF-I and IL-15 gene transfer on the structure and function of skeletal muscles of mdx dystrophic mice 90P
J.D. Schertzer and G.S. Lynch, Basic and Clinical Myology Laboratory, Department of Physiology, The University of Melbourne, VIC 3010, Australia.
The location of the picrotoxin binding site in the glycine receptor pore 91P
Z. Yang, A. Ney and J.W. Lynch, School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia.
ATP requirements of mammalian skeletal muscle Na+/K+-ATPase 92P
T.L. Dutka and G.D. Lamb, La Trobe University, Department of Zoology, Victoria 3086, Australia.
Non-thermal sweating in humans: an investigation of mental sweating from non-glabrous skin surfaces 93P
C.A. Machado-Moreira, J.N. Caldwell, A.K. Meijer, F. Wilmink and N.A.S. Taylor, School of Health Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
Engaging guided-inquiry laboratory classes 94P
K.L. Colthorpe and H.G.G. Ernst, School of Biomedical Sciences, University of Queensland, St. Lucia, Qld, Australia.
Elevated O2• − production at 37°C reduces membrane excitability in isolated rat skeletal muscle 95P
J.N. Edwards,1 W.A. Macdonald,2 C. Van Der Poel1 and D.G. Stephenson,1 1Department of Zoology, La Trobe University, Melbourne 3086, Australia and 2Institute of Physiology and Biophysics, University of Aarhus, Denmark.
Glycerotoxin stimulates exocytosis and endocytosis by increasing intracellular Ca2+ in N-type calcium channels expressing cells 96P
S. Cavaignac,1 M. Schenning,1 D. Proctor,1 M. Stafford,1 N. Lavidis,1 G.W. Zamponi,2 G. Schiavo3 and F.A. Meunier,1 1School of Biomedical Sciences, University of Queensland, St Lucia, Qld 4072, Australia, 2Department of Physiology and Biophisics, University of Calgary, Calgary, Alberta, Canada and 3Molecular Neuropathobiology Laboratory, Cancer Research UK, London Research Institute, Lincoln's Inn Field Laboratories, London, UK.
Calpains in skeletal muscle 97P
R.M. Murphy, E. Verburg and G.D. Lamb, Department of Zoology, La Trobe University, Victoria 3086, Australia.
Glycerotoxin synchronises spontaneous quantal neurotransmitter release independently of action potentials 98P
M.P. Schenning,1 N.A. Lavidis,1 G. Schiavo2 and F.A. Meunier,1 1School of Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia and 2Molecular Neuropathobiology Laboratory, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, London, UK.
Differential regulation of the Nedd4-2 ubiquitin ligase during neurite outgrowth 99P
P.J. Donovan and P. Poronnik, School of Biomedical Sciences, The University of Queensland, St Lucia, Qld, Australia.
The effect of elbow flexion speed and partial limb occlusion on EMG activity 100P
J.A. Sampson,1 H. Groeller,1 D. McAndrew,2 R. Achttien3 and J. Stege,3 1School of Health Sciences, University of Wollongong, NSW, Australia, 2Graduate School of Medicine, University of Wollongong, NSW, Australia and 3University of Maastricht, Netherlands.
Effects of Praescent (plant derived odour) on chronic stress 101P
P.T. Choy,1 G. Haddadan,1 C.N. Poyton,1 R. Einstein2 and N.A. Lavidis,1 1Synaptic Biology Group, School of Biomedical Sciences, The University of Queensland, Brisbane, Qld, Australia and 2Department of Pharmacology, University of Sydney, NSW 2006, Australia.
Contractile performance of isolated muscle from mice over-expressing uncoupling protein 3 and knock-outs 102P
D.C. Bickham1,2 and N.A. Curtin,1 1Imperial College London, Biological Nanosciences, Sth Kensington SW7 2AZ, UK and 2University of Southern Queensland, Centre of Systems Biology, Toowoomba, QLD 4350, Australia.
Functional analysis of novel mutations associated with Hartnup disorder 103P
S. Kowalczuk,1 D.N. Azmanov,2 C.G. Bailey,3 J.E.J. Rasko,3,4 J.A. Cavanaugh2 and S. Bröer,1 1School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia, 2Medical Genetics Research Unit, Medical School, Australian National University, Canberra, Australia, 3Centenary Institute of Cancer Medicine & Cell Biology, University of Sydney, Australia and 4Sydney Cancer Centre, Royal Prince Alfred Hospital, Australia.
Modulation of synaptic transmission by omega-conotoxins CVID and CVIB in spinal superficial dorsal horn 104P
L. Motin, R.J. Lewis and D.J. Adams, School of Biomedical Sciences, University of Queensland, St Lucia, Qld, Australia.
Periconceptional undernutrition alters subpopulations of corticotrophs in the fetal sheep pituitary 105P
K. Farrand,1 S. Maclaughlin,2 S. Tanaka,3 J. Schwartz1 and I.C. Mcmillen,2 1University of Adelaide, Adelaide, SA, Australia, 2University of South Australia, Adelaide, SA, Australia and 3Shizuoka University, Shizuoka, Japan.
Microarray analysis of red and white muscle in the blue swimmer crab, Portunus pelagicus 106P
A.L. Parkinson,1 A.V. Kuballa1,2 and A. Elizur,1,2 1Faculty of Science, Health & Education, University of the Sunshine Coast, Maroochydore Queensland 4558, Australia and 2Department of Primary Industries and Fisheries, Bribie Island Aquaculture Research Centre, Queensland 4507, Australia.
Is neonatal myocardial apoptosis a prelude to cardiac hypertrophy in the hypertrophic heart rat? 107P
E.R. Porrello,1,2 C.L. Curl,1 S.B. Harrap,1 W.G. Thomas2 and L.M.D. Delbridge,1 1Department of Physiology, The University of Melbourne, Parkville, Victoria 3010, Australia and 2Baker Heart Research Institute, Prahran, Victoria 3004, Australia.
Non-specific gap junction action of connexin-mimetic peptides in the rat basilar artery 108P
R.E. Haddock,1,2 K.R. Meaney,1 S.L. Sandow2 and C.E. Hill,1 1John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia and 2School of Medical Sciences, University of New South Wales, NSW 2052, Australia.
Intensity-dependent effect of body tilt angle on calf muscle fatigue in humans 109P
S. Green1,2 and M. Egana,2 1School of Biological, Biomedical and Molecular Sciences, University of New England, Armidale, NSW, Australia and 2Department of Physiology, Trinity College Dublin, Dublin, Ireland.
Expression of P2X7 receptors in primary hippocampal glia: a possible role in cell proliferation and activation 110P
M. Monif,1 C. Reid,2 K. Powell3 and D.A. Williams,1 1Department of Physiology, The University of Melbourne, Vic, Australia, 2Howard Florey Institute, Melbourne, Vic, Australia and 3Department of Medicine, The University of Melbourne, Vic, Australia.
ATP induces a conformational change in the C-terminus domain of the voltage gated skeletal muscle chloride channel (ClC-1) 111P
P.L.Y. Fung and A.H. Bretag, Centre for Advanced Biomedical Studies, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
Effect of mutations on common gating in human ClC-1 chloride channels 112P
J. Cederholm,1 G. Rychkov,1,2 M. Duffield2 and A. Bretag,1 1Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia and 2Physiology Discipline, University of Adelaide, SA 5005, Australia.
Symposium (non-AuPS): Cytoskeleton
The cytoskeletal tropomyosin, Tm5NM1, associates with T-tubules in skeletal muscle and has a role in skeletal muscle contractile function 113P
Anthony Kee1, Nicole Vlahovich1, Gordon Lynch2, Bernadette Vhrovski3, Frances Lemckert3, Robert Parton4, Peter Gunning3, Edna Hardeman1. 1Muscle Development Unit, Children's Medical Research Institute, Westmead, Australia, 2Department of Physiology, University of Melbourne, Australia, 3Oncology Research Unit, Children's Hospital at Westmead, Australia and 4Institute for Molecular Biosciences, University of Queensland, Brisbane, Australia.
Symposium (non-AuPS): Spatial-temporal regulation of cell signalling
Heterogeneity in vascular endothelial KCa expression: relationship to function? 114P
S.L. Sandow, School of Medical Sciences, University of New South Wales, NSW 2052, Australia.
Symposium (non-AuPS): Membrane proteins
Insights into the structural basis of inactivation of HERG K+ channels 115P
J.I. Vandenberg1,2,3, A.P. Hill,1,2 P. Ju,1 M. Sunde,3 A.M. Torres3 and P.W. Kuchel,3 1Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia, 2University of New South Wales, NSW 2052, Australia and 3University of Sydney, NSW 2006, Australia.
 

Author Index


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